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OALib Journal期刊
ISSN: 2333-9721
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深海沉积物宏基因组文库中产蛋白酶克隆的筛选及性质分析
DOI: 10.3724/SP.J.1145.2009.00507, PP. 507-510
Keywords: 深海沉积物,宏基因组文库,蛋白酶,低温酶
Abstract:
提取东太平洋深海沉积物宏基因组dna，构建了未培养微生物宏基因组文库.该文库平均插入片段30kb以上，含有7000个克隆，含外源dna总长度约为210mb.从文库中筛选到18个产胞外蛋白酶的克隆，16srrna分析表明，它们分别来源于假单胞菌(pseudomonas)、寡养单胞菌(stenotrophomonas)和弧菌(vibrio)3个菌属.根据活性大小及来源菌株的16srrna序列比较，选择10个蛋白酶进行酶学性质分析，结果表明，它们的最适作用ph值在7~10之间，最适作用温度在10~40℃之间.其中pro20蛋白酶最适作用温度最低，为10℃；pro1蛋白酶具有最适作用温度低(20℃)、ph耐受性好、热稳定性较好等特性，具有良好的应用开发潜力.图6参16
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