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OALib Journal期刊
ISSN: 2333-9721
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簇毛麦(dasypyrumvillosum)ga20ox基因的分离和鉴定
DOI: 10.3724/SP.J.1145.2009.00479, PP. 479-482
Keywords: 簇毛麦,赤霉素,ga20ox,rt-pcr,原核表达
Abstract:
通过rt-pcr技术克隆了簇毛麦(dasypyrumvillosum)赤霉素生物合成关键酶ga20ox基因的全长序列，命名为dvga20ox，genbank登录号为eu142949.该基因全长1080bp，推测编码359个氨基酸的蛋白质，具有植物ga20ox基因的典型保守结构域lpwket和nyypxcqkp.该基因推导编码蛋白质与小麦、大麦和黑麦草ga20ox蛋白的同源性分别为98%、97%和91%.该序列重组到原核表达载体pet-32a(+)获得的重组子pet-32a(+)-dvga20ox转化大肠杆菌bl21plyss后，iptg诱导表达，sds-page分析表明，dvga20ox基因在大肠杆菌中获得了高效表达，融合蛋白相对分子质量为55×103，与理论值相符.结果为深入研究dvga20ox蛋白的结构与功能以及该基因与株高发育的关系奠定了基础.图3参19
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