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OALib Journal期刊
ISSN: 2333-9721
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一种提取富含油脂植物组织总rna的改良sds酸酚法
DOI: 10.3724/SP.J.1145.2009.00284, PP. 284-287
Keywords: 总rna,麻疯树,油脂,sds酸酚法,胚乳
Abstract:
采用常规的方法提取富含油脂的植物组织总rna常难以取得满意的结果.对林莎两次裂解法进行了改良，即将sds的浓度降低至1%，所用酸酚的ph值降低至4.0，去掉提取液ii处理步骤，获得的总rna效果较好：得率高，rna产量可达74.7μg(100mg)-1；没有dna污染，a260nm/a280nm值为2.026，a260nm/a230nm值为2.082，说明蛋白质及其它污染非常低，通过rt-pcr成功地扩增出了麻疯树curcin基因长为927bp的特异条带.相比林莎的方法，改良sds酸酚法具有经济、简便、适用性广的特点.图5表1参13
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