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OALib Journal期刊
ISSN: 2333-9721
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几株紫色非硫细菌的分离鉴定与产氢特性分析
DOI: 10.3724/SP.J.1145.2009.00120, PP. 120-124
Keywords: 紫色非硫细菌,鉴定,生理生化特性,光合产氢,沼泽红假单胞菌,类球红细菌
Abstract:
从重庆地区不同环境淤泥、泥水样品中，经富集培养、分离纯化，获得5株紫色非硫细菌.根据菌体的菌落形态、染色特性、生理生化特征及活细胞光吸收峰对菌株进行常规鉴定，结合菌株16srdna扩增测序进行分子生物学分析验证，构建了菌株与数据库中近缘菌株的系统发育树.以优化的培养条件（营养、ph、接种量等参数）对供试菌株的生理生化特性和产氢能力做了比较分析.结果显示，5个菌株均为光合产氢细菌，菌株an1、d1为沼泽红假单胞菌(rhodopseudomonaspalustris)，an2、as1、bs1等3株为类球红细菌(rhodobactersphaeroides)；其中类球红细菌菌株an2在给定的培养条件下光合产氢能力最高，可达9.55μg/mld-1，是一株有应用前景的光合产氢细菌.图4表2参15
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