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OALib Journal期刊
ISSN: 2333-9721
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急性冷暴露对仔猪血浆中il-2、il-6、acth和皮质醇水平的影响
DOI: 10.3724/SP.J.1145.2009.00091, PP. 91-94
Keywords: 冷暴露,仔猪,il-2,il-6,acth,皮质醇
Abstract:
为了探讨急性冷应激对仔猪免疫系统的影响，本试验将18头45d“约克夏”仔猪随机分成常温对照组(control,c)、冷暴露组(coldexposure,ce)和冷暴露并阻断糖皮质激素受体组(coldexposure+ru486,cer).常温组在18~20℃、冷暴露各组在4~8℃环境下分栏饲养.冷暴露时间持续12h，在冷暴露开始后的第0、1、3、6、9、12h六个时间点对18头仔猪采血并分离血浆，分别使用双抗体夹心elisa法检测各血浆样品的il-2和il-6水平，放射免疫分析法测定血浆样品的acth和皮质醇水平.结果显示：单纯冷暴露组il-2水平和il-6水平在冷暴露6h时明显升高(p＜0.01)，acth水平在各时间点均明显降低(p＜0.01)，而cortisol水平仅在冷暴露3h时明显升高(p＜0.01)，il-2与il-6的水平呈强相关(r=0.9595)，acth与il-2的水平呈中等强度负相关(r=-0.4359)、与il-6的水平呈弱的负相关，皮质醇与il-2的水平呈弱的负相关、与il-6的水平呈中等强度负相关(r=-0.4478)；受体阻断组il-2在冷暴露6h、9h和12h时均明显升高(p＜0.01)，il-6在冷暴露6h明显升高(p＜0.01)，acth在冷暴露各时间点均明显降低(p＜0.01)，il-2和il-6水平呈中等相关(r=0.4161)，acth与il-2、il-6的水平均呈强负相关(r=-0.6765/-0.7174)，皮质醇与il-2、il-6均呈弱负相关.上述结果表明，急性冷暴露能使il-2、il-6及皮质醇的分泌增强，且阻断糖皮质激素受体会导致il-2和il-6之间以及acth与il-2/il-6之间的相关性改变.图1参21
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