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OALib Journal期刊
ISSN: 2333-9721
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ncrna伴侣分子hfq基因缺失对单核细胞增生李斯特菌环境适应能力的影响*
DOI: 10.3724/SP.J.1145.2015.03044, PP. 1-7
Keywords: 核细胞增生李斯特菌,rna,hfq基因,同源重组,分子伴侣
Abstract:
hfq作为一种保守的rna分子伴侣蛋白，主要通过与ncrna结合来参与调控单核细胞增生李斯特菌（lm）的生命活动。本研究利用基因重叠延伸pcr（soe-pcr）方法构建具有氯霉素抗性的pksv7-δhfq重组穿梭质粒，电转化至lm-sb5感受态细胞后在42℃和氯霉素压力下进行同源重组，筛选lm？sb5hfq基因缺失株，并分析hfq基因缺失株在不同温度、ph、高盐、乙醇、h2o2、tritonx-100胁迫环境下的适应能力。结果成功构建得到一株遗传性稳定的lmhfq基因缺失株lm-δhfq；lm-δhfq对的适应能力显著降低，对30℃、37℃、42℃、ph4、9及tritonx-100的适应能力没有显著变化表明参与了lm对高盐、乙醇、h2o2胁迫环境的适应过程。本研究为进一步揭示hfq蛋白在参与ncrna调控细菌生命活动的机制奠定了基础。
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