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OALib Journal期刊
ISSN: 2333-9721
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木质素磺酸盐好氧降解菌的筛选及降解特性研究*
DOI: 10.3724/SP.J.1145.2015.05028, PP. 1-8
Keywords: 木质素磺酸盐,细菌降解,分离筛选,降解特性
Abstract:
木质素是结构复杂的难降解有机物，研究其微生物降解机制对解决制浆造纸黑液处理问题，促进木质纤维素的再生利用具有重要意义。本文从制浆造纸厂活性污泥样本中分离出5株以木质素磺酸盐为唯一碳源和能源的好氧细菌。选取降解性能优异的菌株，考察温度、ph、碳氮比对该菌株生长和降解效果的影响，并对降解后木质素的官能团、元素组成变化和降解产物进行分析，推测木质素降解机制。结果显示：5株细菌均可有效降解木质素，其中菌株sphingobacteriumsp.hy-h的降解能力最强。通过正交试验确定菌株hy-h的最佳处理条件为：温度30℃，ph=7.0，碳氮比5:1。在此条件下，菌株hy-h对木质素磺酸盐（1g？l-1）的5d降解率为30.6%。降解后木质素的酚羟基和甲氧基含量减少，羰基和羧基含量增加。研究表明，木质素降解过程中主要发生了α羟基氧化、β-o-4键断裂、脱甲基及脱羧作用，最终生成co2。图3表5参24
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