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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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基于缺口酶的链置换等温扩增技术
DOI: 10.3724/SP.J.1145.2015.03039, PP. 1-8
Keywords: 缺口酶,等温扩增,剪切,链置换扩增
Abstract:
传统的链置换等温扩增技术中需要加入修饰的底物，为了实现常规底物下的链置换等温扩增技术，本研究采用缺口酶nt.bstnbi代替传统链置换等温扩增技术中的限制性内切酶，通过优化反应条件和引物序列，实现了常规底物下的链置换等温扩增，成功解决了传统方法中的这一技术难题，建立了一种环保、低成本的新型链置换等温扩增技术，该种方法可以检测到2pmol/l的质粒dna。扩增反应在15-30min即可完成，并且产物可通过琼脂糖凝胶电泳进行直接检测，更为简便、快捷，具有非常强的实际应用潜力，特别适用于现场检测。由此，我们建立起了基于缺口酶的链置换等温扩增体系。
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