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OALib Journal期刊
ISSN: 2333-9721
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青枯菌野生型菌株和无毒突变株细胞表面的吸附特性[英文]
DOI: 10.3724/SP.J.1145.2010.00499, PP. 499-503
Keywords: 青枯菌,野生型,无毒突变株,吸附特性,色谱行为
Abstract:
应用高效离子交换色谱和激光光散射仪检测器（hplc/malls）研究青枯菌野生型菌株79#和无毒突变菌株rs52’对强阴离子交换树脂（superq-650c）的吸附特性.结果显示，这2个菌株在色谱行为和吸附特性方面差异显著.静态吸附试验结果也表明，在相同条件下，79#菌株的吸附速率和吸附量与rs52’菌株不同，79#菌株在60min达到吸附平衡，最大吸附量为1.09×109cfu/g(干树脂)；rs52’菌株在120min达到吸附平衡，最大吸附量为2.10×109cfu/g(干树脂).进一步采用langmuir和freundlich吸附等温线方程比较79#菌株和rs52’菌株与树脂之间的吸附能力，研究结果与静态吸附试验结果以及高效离子交换色谱的分析结果相一致，rs52’菌株与树脂之间显示出更强的吸附力；此外，用freundlich方程拟合的效果优于langmuir方程，拟合的曲线与实验数据更吻合.图4表2参16
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