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盐生杜氏藻对硝酸盐的转运特性

DOI: 10.3724/SP.J.1145.2010.00489, PP. 489-492

Keywords: 盐生杜氏藻,硝酸盐,硝酸盐转运蛋白,硝酸盐吸收率

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Abstract:

盐生杜氏藻(dunaliellasalina)是已知最耐盐的真核光合生物,硝酸盐作为盐生杜氏藻氮源的主要来源,影响盐生杜氏藻的生长代谢,研究盐生杜氏藻对硝酸盐的转运吸收状况,可为生长于高盐、低盐环境中作物吸收氮的研究提供理论基础.以水杨酸法测定硝酸盐浓度,设计相关实验,结果发现盐生杜氏藻在低浓度的硝酸盐(0.2mmol/l)溶液中对硝酸盐有吸收,吸收效率随硝酸盐浓度的增大而提高;当盐浓度为2mol/l时其硝酸盐吸收效率最高,说明一定的盐浓度能诱导盐生杜氏藻对硝酸盐的吸收;经nh4+预处理过的盐生杜氏藻,在相同硝酸盐浓度下培养时其硝酸盐吸收效率比未经nh4+预处理的低.实验结果提示盐生杜氏藻存在高亲和性的硝酸盐转运蛋白.图6参17

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