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OALib Journal期刊
ISSN: 2333-9721
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酵母双杂交筛选oipk相互作用蛋白
DOI: 10.3724/SP.J.1145.2010.00474, PP. 474-477
Keywords: 酵母双杂交,ser/thr蛋白激酶,植物抗病性,壳寡糖,oipk
Abstract:
前期研究发现烟草中一个壳寡糖诱导的ser/thr蛋白激酶（oligochitosaninducedproteinkinase,oipk）在壳寡糖诱导的烟草抗性反应中起着重要作用.目前在烟草中发现oipk基因的两个选择性剪接体：oipkl和oipks，其中oipkl是3个选择性剪接体中序列最长的.为进一步研究oipk在壳寡糖诱导植物抗性中起的作用，本研究采用酵母双杂交方法，以pgbkt7-oipkl为诱饵质粒，筛选构建在pact2载体上的烟草cdna文库，寻找oipkl的相互作用蛋白.结果表明oipkl蛋白在酵母中可以和烟草的s-腺苷甲硫氨酸合成酶、泛素激活酶e1、丙酮酸激酶、过氧化物酶相互作用.实验结果为研究壳寡糖诱导抗性机理提供了一定依据.图3参26
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