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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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抗氧化系统在蜈蚣草细胞砷解毒机制中的作用
DOI: 10.3724/SP.J.1145.2010.00453, PP. 453-456
Keywords: 蜈蚣草,抗氧化系统,砷污染,砷富集,砷解毒
Abstract:
砷超富集植物――蜈蚣草体内可以富集高浓度的砷且不表现出受害症状，暗示蜈蚣草细胞具有较强的砷解毒机制.为探讨抗氧化系统在蜈蚣草砷解毒机制中的作用，测定了0、0.5、1和2mmol/l的砷酸盐[as(v)]vv)]、亚砷酸盐[as(iii)]和二甲基胂酸盐（dma）处理下蜈蚣草愈伤组织的丙二醛（malonyldiadehydealonyldiadehyde，mda）含量、非酶类抗氧化物质含量和酶类抗氧化物质的活性.结果表明，0.5mmol/las(vv)和dma就可以诱导氧化胁迫的产生，浓度越高胁迫程度也越严重.而1mmol/l的as(iii)仍未使蜈蚣草愈伤组织产生氧化胁迫.在as(iii)处理下，酸溶性巯基含量增加，叶绿素和类胡萝卜素含量不变，过氧化氢酶（catalase，cat）和过氧化物酶（peroxidase，pod）活性增加.在as(处理下，酸溶性巯基含量减少，叶绿素和类胡萝卜素含量略有上升；cat和pod活性先增加后减少.在dma处理下，酸溶性巯基含量减少，叶绿素含量降低而类胡萝卜素含量增加，cat和pod活性先增加后减少.与as(和dma相比，as(iii)处理下高酸溶性巯基含量、高氧化酶活性和低mda含量暗示as(iii)对蜈蚣草的毒性可能比as(和dma要小.这些结果表明，酸溶性巯基和酶类抗氧化物质（cat和pod）在蜈蚣草细胞砷解毒机制中发挥着重要作用.图4参22
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