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纤维素酸解副产物对clostridiumacetobutylicumcicc8012发酵的影响

DOI: 10.3724/SP.J.1145.2010.00845, PP. 845-850

Keywords: 纤维素,酸水解,抑制物,clostridiumacetobutylicumcicc8012,丁醇发酵

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Abstract:

为确定纤维素酸解副产物对c.acetobutylicumcicc8012发酵生产纤维燃料丁醇的影响,选择乙酸根、甲酸、葡萄糖醛酸、氯化钠、硫酸钠、糠醛、羟甲基糠醛(hmf)、香豆酸、阿魏酸、丁香醛、香草醛等纤维素酸解副产物,研究其对菌种发酵的影响.结果表明:甲酸和酚类物质的抑制作用强烈,0.5g/l的甲酸、阿魏酸或香豆酸存在时对丁醇的抑制率分别为88.1%、22.1%、82.4%,丁香醛(≥0.5g/l)、香草醛(≥2g/l)可使发酵的延滞期变长,但抑制作用小于阿魏酸和香豆酸;1g/l的糠醛、hmf、葡萄糖醛酸存在时对丁醇的抑制率分别为9.8%、4.6%、7.3%,10g/l乙酸钠存在时对丁醇的抑制率为4.8%,这4种物质对丁醇发酵的抑制作用不大;硫酸钠、氯化钠质量浓度大于5g/l时对发酵产生抑制作用,浓度达到20g/l时菌体几乎不能生长.当各抑制物混合时,若抑制物的含量超过临界点,各抑制物间存在相互促进的作用;小麦秸秆水解液的发酵结果表明,水解液成分复杂,抑制作用更为强烈.图3表7参20

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