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OALib Journal期刊
ISSN: 2333-9721
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基于均匀设计优化新鞘氨醇菌us6-1对高分子量多环芳烃的降解条件
DOI: 10.3724/SP.J.1145.2010.00875, PP. 875-878
Keywords: 新鞘氨醇菌,均匀设计,高分子量多环芳烃（hmw-pahs）,生物降解,接种量
Abstract:
运用均匀设计优化一株新鞘氨醇菌novosphingobiumpentaromativoransstainus6-1对芘、荧蒽、苯并[a]芘等高分子量多环芳烃（highmolecularweighpolycyclicaromatichydrocarbons，hmw-pahs）的降解条件.结果表明，在最适生长条件（30℃、ph6.5及nacl浓度为2.5%）下，接种量与底物浓度是影响该菌降解能力的关键因素.在接种量d660nm为0.6、芘初始浓度56mgl-1、培养时间5d的情况下，菌株us6-1对芘的降解达到32.5mgl-1，预测平均准确率达99.38%；在接种量d660nm为0.6、荧蒽初始浓度48mgl-1、培养时间5d的情况下，对荧蒽的降解达到34.3mgl-1，预测平均准确率达99.61%；在接种量d660nm为0.1、苯并[a]芘初始浓度60mgl-1、培养时间为5d时，对苯并[a]芘的降解达到24mgl-1，预测平均准确率达98.75%.菌株us6-1对芘及苯并[a]芘的降解能力比未优化前分别提高了29.2%与58%.图1表5参21
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