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OALib Journal期刊
ISSN: 2333-9721
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一株嗜酸性产纤维素酶真菌的特性及产酶条件优化
DOI: 10.3724/SP.J.1145.2010.00863, PP. 863-869
Keywords: 嗜酸,纤维素酶,土曲霉,系统发育分析,响应面法,最优培养条件
Abstract:
从四川海螺沟原始森林腐土中分离到一株嗜酸性产纤维素酶的真菌x-13，其主要特点是产纤维素酶的最适ph及其纤维素酶最适反应ph均为2.0.在pda培养基上培养时菌落呈浅黄色至肉桂色，反面呈黄色至棕褐色，产黄色色素；菌丝体透明有隔膜，分生孢子呈球形或近球形.根据菌株的形态特征以及its序列同源性和系统发育分析结果，鉴定该菌株为土曲霉（aspergillusterreusthom）.该菌最佳产酶培养时间为8~10d；最适产酶温度为30℃，纤维素酶最适反应温度为50℃；最佳碳源、氮源分别为纤维素粉和硫酸铵.通过响应面法对菌株产纤维素酶条件进行优化，使菌株x-13纤维素酶活从1.39iu/ml提高到2.94iu/ml，提高了111.5%.图10表7参15
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