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OALib Journal期刊
ISSN: 2333-9721
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泸州古酿酒窖池中两株产甲烷杆菌比较研究
DOI: 10.3724/SP.J.1145.2010.00840, PP. 840-844
Keywords: 产甲烷菌,比较研究,新种分离,窖泥,16srdna序列,系统发育分析,泸州老窖
Abstract:
采用厌氧操作技术，从泸州老窖古酿酒窖池窖泥中分离到两株产甲烷杆菌0372-d1和0072-d2.0372-d1菌体形态为长杆状，略弯，两端整齐，不运动，可由多个菌体形成长链；在固体培养基中难以长出菌落，只利用h2+co2产生甲烷.0072-d2菌体形态为弯曲杆状，淡黄色圆形菌落，利用h2+co2或甲酸盐作为唯一碳源生长.两株菌最适生长温度均为35℃、菌株0372-d1最适生长ph为6.5~7.0，生长ph范围5.0~8.0；菌株0072-d2最适生长ph则为7.5.在各自最适条件下培养，两株菌的最短增代时间分别为19h和8h.通过形态、生理生化特征和16srdna序列的同源性分析，表明菌株0372-d1为产甲烷杆菌属的一个新种.0072-d2则为甲酸甲烷杆菌（methanobacteriumformicicum）的新菌株，相似性为99%.图6表1参16
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