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OALib Journal期刊
ISSN: 2333-9721
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麻疯树叶片高效再生体系与不定芽起源
DOI: 10.3724/SP.J.1145.2010.00789, PP. 789-793
Keywords: 麻疯树,叶片,再生,植物生长调节剂,不定芽,愈伤组织
Abstract:
建立了培养周期短、再生效率高的麻疯树叶片再生体系.6-ba是诱导麻疯树叶片产生愈伤组织的关键外源激素，添加低浓度的2,4-d能增强6-ba的诱导效果.最佳愈伤组织诱导培养基是ms+2.0mg/l6-ba+0.05mg/l2,4-d，瘤状愈伤组织的诱导率高达91.98%±2.1%.光培养下产生的愈伤组织质地紧密，瘤状结构丰富，再生能力强.随着继代次数的增加，愈伤组织褐化加重，逐渐失去再生能力.不定芽再生培养基中，比较了3种细胞分裂素6-ba、kt和tdz对诱导愈伤组织分化不定芽的影响.6-ba诱导不定芽的能力最优，其次是tdz，kt最差.kt与ba的组合诱导不定芽再生的能力最强，最适的不定芽再生培养基为ms+0.5mg/l6-ba+0.5mg/lkt+0.1mg/liba+0.1mg/lga3，再生率高达68.01%±2.5%，平均每个愈伤组织分化出4.25个芽.70%的再生苗在ms培养基添加0.2mg/liba或naa（0.1或0.2mg/l）中均能产生根系，平均根系5.2个.对麻疯树叶片再生的各阶段进行了石蜡切片，揭示了不定芽的起源及发育的全过程，明确了不定芽起源于叶片的表皮细胞层.图3表3参30
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