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OALib Journal期刊
ISSN: 2333-9721
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黄曲霉培养条件的优化及黄曲霉毒素b1的提取
DOI: 10.3724/SP.J.1145.2010.00724, PP. 724-729
Keywords: 黄曲霉,黄曲霉毒素b1,大米培养基,温度,湿度,优化
Abstract:
为提取高浓度的黄曲霉毒素b1，以大米为培养基培养黄曲霉，分别在不同温度梯度（15℃、20℃、25℃、30℃、35℃）和湿度梯度（0%、80%、85%、90%和95%和100%）下培养，每天测量一次菌斑直径的变化（共测18次），然后采用logistic（一级）和ratkowsky（二级）模型对黄曲霉菌斑大小进行拟合和检验，最后借助响应面模型对黄曲霉产毒条件进行优化.结果显示，在35℃和100%湿度的培养条件下，只需培养16~17d即可获得直径较大的黄曲霉菌斑，提取黄曲霉毒素b1浓度为3312ng/ml，单位面积黄曲霉毒素b1产量是优化前（1101.471ng/ml）的3倍.图5表4参18
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