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OALib Journal期刊
ISSN: 2333-9721
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一株黑松-美味牛肝菌菌根辅助细菌的筛选及鉴定
DOI: 10.3724/SP.J.1145.2010.00701, PP. 701-704
Keywords: 黑松,美味牛肝菌,根际,菌根辅助细菌,蜡状芽孢杆菌
Abstract:
为探讨菌根际有益微生物之间的作用关系及其互作对黑松（pinusthunbergii）生长的影响，对分离自黑松-美味牛肝菌（boletusedulis，be）和黑松-黄色须腹菌（rhizopogenluteous）菌根际土壤的81株细菌进行菌根辅助细菌（mycorrhizahelperbacteria,mhb）的筛选.通过干皿对抗法筛选获得1株具mhb潜力的菌株hb12，hb12菌株对be菌丝生长的平均增长率达34.4%.盆栽试验结果表明，单接be处理的黑松根系菌根侵染率为36.8%，而双接种be和hb12菌株处理的菌根侵染率为54.5%；双接种be和hb12处理、单接be处理和单接hb12处理较未接种处理的黑松苗高提高44%、36%和24.5%，地径提高27.7%、22.9%和26.5%.由此可确定菌株hb12为黑松-美味牛肝菌的菌根辅助细菌.经形态观察、biolog系统鉴定及16srrna分子鉴定，初步确定菌株hb12为蜡状芽孢杆菌（bacilluscereus）.图2表2参19
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