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OALib Journal期刊
ISSN: 2333-9721
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云南腾冲热泉极端嗜酸热硫化叶菌多样性研究
DOI: 10.3724/SP.J.1145.2010.00692, PP. 692-696
Keywords: 硫化叶菌,嗜酸热古菌,系统进化分析,多样性,腾冲
Abstract:
硫化叶菌（sulfolobus）是极端嗜热古菌遗传机制研究的重要模式菌株，广泛分布于酸性热泉中.对分离自云南腾冲酸性热泉的11株极端嗜酸热菌株进行16srrna基因序列测定，并通过菌体形态观察、16srrna基因可变区序列比较、菌株16srrna基因相似性比较及系统发育分析，研究腾冲热泉硫化叶菌的多样性.结果表明，分离自腾冲热泉的11株硫化叶菌的16srrna基因序列与分离自世界上其它地区的8个硫化叶菌的标准菌株的相似性为85.8%~94.9%，与同样分离自腾冲热泉的s.tengchongensis标准菌株的相似性为96.6%~97.5%，在分类学上具有形成新种的可能.在16srrna基因高可变区序列上，与分离自世界上其它地区的8个硫化叶菌的标准菌株存在明显的差异.系统发育分析表明，分离自腾冲热泉的硫化叶菌在系统发育树上形成两个亲缘关系较为紧密的簇群，可见腾冲热泉硫化叶菌不仅具有一定的多样性，同时具有较明显的地域性特征.图2表3参16
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