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OALib Journal期刊
ISSN: 2333-9721
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黄芪多糖的提取及按相对分子质量分段分离
DOI: 10.3724/SP.J.1145.2010.00719, PP. 719-723
Keywords: 黄芪多糖,渗滤提取,分步醇沉,凝胶层析,高效渗透凝胶色谱
Abstract:
黄芪多糖（aps）具有多种生物活性以及广泛的临床应用，然而目前对其研究大多以相对分子质量（mr）范围分布较大的总多糖为对象，使得药理活性机制等研究难于深入.采用常温渗滤方法提取黄芪多糖，粗多糖得率为15.92%，多糖含量为82.43%，较传统水煮醇沉法有较大提高；对提取的粗多糖用离子交换层析进行纯化、用分步醇沉联合凝胶层析进行分段分离，获得7个多糖组分，经高效液相色谱（hplc）测定，分离的7个组分皆为均一组分，高效凝胶渗透色谱（hpgpc）测定证明该7个组分具有不同的mr，其范围分布在1.4×103~157.7×103，为进一步研究黄芪多糖活性提供了良好的材料.图7表2参21
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