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OALib Journal期刊
ISSN: 2333-9721
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铁皮石斛外源lea3基因的转化及耐盐性分析
DOI: 10.3724/SP.J.1145.2010.00622, PP. 622-626
Keywords: 铁皮石斛,类原球茎（plbs）,lea3基因,基因枪,转化,耐盐性
Abstract:
利用基因枪法将来源于大麦（hordeumvulgarel.）的抗旱耐盐基因lea3导入铁皮石斛（dendrobiumcandidumwall.exlindl）的类原球茎（plbs）中，经膦丝菌素类除草剂ppt筛选2次获得抗性plbs，于无选择压下分化获得不定芽，再经ppt筛选和生根壮苗培养获得转化植株.对转化植株进行除草剂ppt叶片涂抹检测和lea3基因的pcr检测，表明lea3基因已整合到6个株系7株铁皮石斛转化植株基因组中，转化频率为1.05%.与对照相比，获得的转基因植株的耐盐胁迫能力明显增强，表明遗传转化lea3基因可用于石斛抗旱耐盐新品种的选育.图4表1参23
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