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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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土壤rna提取方法研究进展
DOI: 10.3724/SP.J.1145.2011.00918, PP. 918-922
Keywords: 土壤微生物,rna提取方法,裂解,rnase
Abstract:
总rna提取是进行实时荧光定量pcr、northern杂交分析及cdna文库建立等分子生物学实验研究的基础.提取出高质量高产量的土壤rna已成为研究土壤微生物的关键步骤.综述了常用的几种土壤rna提取方法，包括强变性剂法、trizol法、ctab法、sds-phenol法、酶解法以及试剂盒等方法.提取过程中化学试剂的选择及配比需依据不同的土壤类型，其中trizol法在提取总rna时应用广泛，再结合适当的物理破碎及生物学方法，可得到理想的rna.试剂盒法提取土壤rna方便快捷，纯度高但产量低，且对土壤具有选择性.最后提出了土壤rna提取过程常见的一些问题和注意事项，为土壤rna提取方法的选择提供依据.参56
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