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OALib Journal期刊
ISSN: 2333-9721
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一株除草剂氰氟草酯降解菌的分离鉴定与降解特性
DOI: 10.3724/SP.J.1145.2011.00869, PP. 869-875
Keywords: 氰氟草酯,除草剂,微生物降解,寡养单胞菌属,降解产物
Abstract:
氰氟草酯（cyhalofop-buty1）是一种芳氧苯氧羧酸酯类除草剂，其迁移、转化和分解等行为对环境及人类健康具有潜在危害.为了解其微生物代谢机制，从长期使用氰氟草酯的稻田土壤中分离到1株氰氟草酯降解菌株，命名为qdz-b.菌株qdz-b的16srdna基因序列长度为1400bp，在rdp数据库比对结果表明其与寡养食单胞菌属菌株的同源性最近，与模式菌株嗜麦寡养食单胞菌stenotrophomonasmaltophilia（ab008509）的同源性为99.8%.根据表型特征、生理生化特性和16srdna序列同源性分析，将菌株qdz-b鉴定为寡养单胞菌属（stenotrophomonassp.）.接种量为5%时，菌株qdz-b在基础盐液体培养基中5d对100mgl-1氰氟草酯的降解率约为88.2%.菌株qdz-b的降解效果与接种量成正相关，但接种量大于5%时，降解效率趋于平稳.菌株qdz-b降解氰氟草酯的最适温度为30℃，最适ph为7.0.通过质谱分析鉴定了3个菌株qdz-b降解氰氟草酯的代谢产物，分别为氰氟草酸、氰氟草酰胺和2-氟-3-对-乙氧基苯氧基苯酰胺.图11参21
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