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OALib Journal期刊
ISSN: 2333-9721
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黑曲霉耐热木聚糖酶xynb在毕赤酵母中的高效表达
DOI: 10.3724/SP.J.1145.2011.00892, PP. 892-896
Keywords: 黑曲霉,木聚糖酶,毕赤酵母gs115,甲醇,诱导表达
Abstract:
高比活木聚糖酶的高效表达是进一步提高木聚糖酶发酵效价、降低生产成本的有效途径.将黑曲霉木聚糖酶基因xynb（不含信号肽）克隆到分泌型表达载体ppic9k上，线性化后电击转化巴斯德毕赤酵母gs115，g418和pcr鉴定的阳性转化子经0.5%甲醇、在28℃诱导表达.sds-page分析表明，该蛋白相对分子质量为20×103左右.优化的诱导表达条件为，每隔12h添加0.5%的甲醇，发酵5d后，比活达4757u/mg；其最适温度为55℃，最适ph为5.0，80℃处理30min后仍有74%的残余酶活.图9参18
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