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OALib Journal期刊
ISSN: 2333-9721
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白囊耙齿菌腐朽木材相关cdna片段的序列克隆与分析
DOI: 10.3724/SP.J.1145.2011.00860, PP. 860-863
Keywords: 白囊耙齿菌,白桦,木材腐朽,mrna差异显示
Abstract:
为了解白囊耙齿菌腐朽木材的分子机制，利用基因差异显示技术（differentialdisplayreversetranscriptase，pcrddrt-pcr）研究了白囊耙齿菌腐朽白桦木材前后基因表达的差异.共分离出5个腐朽后在菌丝体中特异表达的cdna片段，分别命名为a2、a7、c6、b8和b3.序列同源性比对发现：片段a2、a7和c6分别与编码漆酶（laccase）、纤维素酶a（cellulasea）和麦芽糊精磷酸化酶（maltodextrinphosphorylase）等3个基因具有高度同源性；b8与一个编码响应调节蛋白的基因具有高度同源性.研究结果显示，所筛选的差异表达基因a2、a7和c6等与木材降解有密切关系.图2表2参22
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