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OALib Journal期刊
ISSN: 2333-9721
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深海沉积物微生物宏基因组文库中imp环水解酶的筛选及基因克隆
DOI: 10.3724/SP.J.1145.2011.00855, PP. 855-859
Keywords: 深海沉积物,宏基因组文库,imp环水解酶,保守结构域
Abstract:
为了解嘌呤合成途径的关键酶――imp环水解酶的多样性，从深海沉积物样品中提取总dna构建cosmid宏基因组文库，从中筛选出一个具有imp环水解酶活性的克隆（caimp1）.通过基因步移的方法从caimp1的约35kb的插入片段中扩增出长度为1599bp的完整的imp环水解酶基因，该基因包含mgs和aicarft_impchas两个保守结构域.活性检测结果表明caimp1克隆的发酵液上清具有较强的imp环水解酶活性.该酶与数据库中收录的imp环水解酶的氨基酸序列同源性较低，系统发育分析也表明该酶与其它参照序列亲缘关系较远，表明该序列可能为一个新的imp环水解酶基因.图5表3参17
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