|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
盐胁迫下dslhcb3和cao基因的表达及类囊体磷酸化状态转化
DOI: 10.3724/SP.J.1145.2011.00851, PP. 851-854
Keywords: 盐藻,实时pcr,lhcb3基因,cao基因,磷酸酯酶,类囊体膜
Abstract:
lhcⅱ在在类囊体膜中除了进行光能的吸收和传递之外，在维持类囊体膜的结构，调节激发光能在两个光系统之间的分配，光保护以及对各种环境的适应等过程中都起着重要的作用.为揭示盐藻lhcb在盐胁迫下的作用机制，以盐生杜氏藻（dunaliellasalina）为材料，应用实时pcr的方法和蛋白电泳技术，研究lhcb3和叶绿素a加氧酶cao基因在低盐胁迫下的表达变化，以及盐藻类囊体膜的磷酸化水平和磷酯酶的变化.结果表明，在低盐环境下，lhcb3和cao基因的表达活性都呈降低的总趋势，这可能是盐藻适应不同浓度盐环境胁迫的不同反应.类囊体膜蛋白磷酸化水平先是降低，之后有所升高.同时，发现磷酸酯酶有着相同的变化趋势，并在12h时最高.由此认为低盐条件下，类囊体膜蛋白在前期磷酸化水平的降低与磷酸酯酶活性降低有关，但在12h后磷酸化水平有所升高，可能是细胞内平衡调节的结果，即磷酸激酶活性增强的结果.图5参30
| [1] | 2bennettj.phosphorylationofchloroplastmembranepolypeptides.nature,1977,269:344~346
|
| [2] | 7tillerk,linkg.phosphorylationanddephosphorylationaffectfunctionalcharacteristicsofchloroplastandetioplasttranscriptionsystemsfrommustard.emboj,1993,12:1745~1753
|
| [3] | 8danona,mayfieldsp.adp-dependentphosphorylationregulatesrna-bindinginvitro:implicationsinlight-modulatedtranslation.emboj,1994,13:2227~2235
|
| [4] | 9bennettj.proteinphosphorylationingreenplantchloroplasts.annurevplantphysiol&plantmolbiol,1991,42:281~311
|
| [5] | 10wollmanfa.statetransitionsrevealthedynamicsandflexibilityofthephotosyntheticapparatus.emboj,2001,20:3623~3630
|
| [6] | 11forkdc,satohk.thecontrolbystatetransitionofthedistributionofexcitationenergyinphotosynthesis.annurevplantphysiol,1986,37:335~361
|
| [7] | 12aroem,ohadi.redoxregulationofthylakoidproteinphosphorylation.antioxidredoxsignal,2003,5:55~67
|
| [8] | 13allenjf.statetransitions-aquestionofbalance.science,2003,299:1530~1532
|
| [9] | 14bonaventurac,myersj.fluorescenceandoxygenevolutionformchlorellapyrenoidosa.biochimetbiophysacta,1969,189:366~383
|
| [10] | 15huangq(黄秦),caoy(曹瑜),wup(吴鹏),shiy(史岩),zhangs(张书),qiaodr(乔代蓉),caoy(曹毅).purificationandantibodypreparationandanalysisofdifferentdomainsofgpdhproteinsfromdunaliellasalina.chinjapplenvironbiol(应用与环境生物学报),2009,15(2):202~206
|
| [11] | 16wollmanfa,delepelairep.correlationbetweenchangesinlightenergydistributionandchangesinthylakoidmembranepolypeptidephosphorylationinchlamydomonasreinhardtii.jcellbiol,1984,98:1~7
|
| [12] | 17bonaventurac,myersj.fluorescenceandoxygenevolutionformchlorellapyrenoidosa.biochimbiophysacta,1969,189:366~383
|
| [13] | 18delosmer,olivej,wollmanfa.changesinlightenergydistributionuponstatetransitions:aninvivophotoacousticstudyofthewidetypeandphotosynthesismutantsfromchlamydomonasreinhardtii.biochimbiophysacta,1996,1273:150~158
|
| [14] | 19escoubasjm,lomasm,larochej,falkowskipg.lightintensityregulationofcabgenetranscriptionissignaledbytheredoxstateoftheplastoquinonepool.procnatlacadsciusa,1995,92:10237~10241
|
| [15] | 20liuxd,shenyg.hypoosmoticshockinducesastateitransitionofphotosyntheticapparatusindunaliellasalina.chinscibull,2004,49:672~675
|
| [16] | 21melisa.excitationenergytransfer:functionalanddynamicaspectsoflhc(cab)proteins.in:ortdr,yocumcfeds.oxygenicphotosynthesis:thelightreactions.dordrecht,netherlands:kluweracademicpublishers,1996,48:579~603
|
| [17] | 22björkmano,boadmannk,andersonjm,thornesw,goodchilddj,phliotisna.effectoflightintensityduringgrowthofatriplexpatulaonthecapacityofphotosyntheticreactions,chloroplastcomponentsandstructure.carnegieinstwashyearb,1972,71:115~135
|
| [18] | 23tanakar,koshinoy,sawas,ishiguros,okadak,tanakaa.overexpressionofchlorophyllideaoxygenase(cao)enlargestheantennasizeofphotosystemiiinarabidopsisthaliana.plantj,2001,26:365~373
|
| [19] | 24tatsurum,jürgenewp,anastasiosm.biosynthesisanddistributionofchlorophyllamongthephotosystemsduringrecoveryofthegreenalgadunaliellasalinafromirradiancestress.plantphysiol,2002,128:603~614
|
| [20] | 25allenjf.proteinphosphorylationinregulationofphotosynthesis.biochimetbiophysacta,1992,1098:275~335
|
| [21] | 26delosmer,olivej,wollmanfa.changesinlightenergydistributionuponstatetransitions:aninvivophotoacousticstudyofthewidetypeandphotosynthesismutantsfromchlamydomonasreinhardtii.biochimetbiophysacta,1996,1273:150~158
|
| [22] | 1janssons.thelight-harvestingchlorophylla/b-bindingproteins.biochimbiophysacta,1994,1184:1~19
|
| [23] | 3bennettj.chloroplastphosphoproteins.evidenceforathlakoid-boundphosphoproteinphosphatase.eurjbiochem,1980,104:85~89
|
| [24] | 4foyerch.stromalproteinphosphorylationinspinach(spinaciaoleracea)chloroplasts.biochemj,1985,231:97~103
|
| [25] | 5bhallap,bennettj.chloroplastphosphoproteins:phosphorylationofamr12×103stromalproteinbytheredox-controlledkinasofthylakoidmembranes.archbiochem&biophys,1987,252:97~104
|
| [26] | 6sollj,bennettl.localizationofamr64×103phosphoproteininthelumenbetweentheouterandinnerenvelopesofpeachloroplasts.eurjbiochem,1988,175:301~307
|
| [27] | 27giardimt,conaa,geikenb,kucerat,masojidekj,mattooak.long-termdroughtstressinducesstructuralandfunctionalreorganizationofphotosystemii.planta,1996,199:118~125
|
| [28] | 28turkinamv,vainonenjp,arseniovillarejo,venerav.co2limitationinducesspecificredox-dependentproteinphosphorylationinchlamydomonasreinhardtii.proteomics,2006,6:2693~2704
|
| [29] | 29hagitz,itzhako.light,redoxstate,thylakoid-proteinphosphorylationandsignalinggeneexpression.trendsbiochemsci,2003,28:467~470
|
| [30] | 30pursiheimos,mulop,rintamäkie,aroem.coregulationoflight-harvestingcomplexiiphosphorylationandlhcbmrnaaccumulationinwinterrye.plantj,2001,26(3):317~327
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
