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OALib Journal期刊
ISSN: 2333-9721
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盐藻lhcb3蛋白的表达纯化及抗体制备
DOI: 10.3724/SP.J.1145.2011.00847, PP. 847-850
Keywords: 盐生杜氏藻,光系统ⅱ,光捕获蛋白,表达纯化,抗体
Abstract:
为了解盐生杜氏藻（dunaliellasalina）主要光捕获蛋白lhcb蛋白的功能，将已获得的盐藻lhcb3基因构建到原核表达载体pet32a-dslhcb3，通过优化表达条件，建立了高效的重组系统.pet32a-dslhcb3在大肠杆菌中的优化表达条件为1mmol/liptg在37℃下诱导4h.采用镍离子亲合层析纯化获得lhcb3蛋白，并以此为抗原制备了多克隆抗体，经琼脂糖扩散检测效价，在1:16处有明显沉淀.提取盐藻总蛋白，经过制备的lhcb3抗体杂交，在29000处获得两条明显的杂交条带，为进一步研究盐藻lhcⅱ蛋白表达机理奠定了基础.图4参16
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