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OALib Journal期刊
ISSN: 2333-9721
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nacl与cd对小球藻光系统ⅱ（psⅱ）活性的影响
DOI: 10.3724/SP.J.1145.2011.00839, PP. 839-846
Keywords: 蛋白核小球藻,盐胁迫,镉胁迫,叶绿素荧光,光系统ⅱ（psⅱ）
Abstract:
运用叶绿素荧光技术分析了盐胁迫和重金属镉（cd）对蛋白核小球藻（chlorellapyrenoidosa）光系统ⅱ（psⅱ）的影响机理.实验中小球藻培养基设置nacl浓度梯度分别为0、25、100mmol/l，然后在不同nacl浓度的培养基中再加入cdcl2溶液，使培养基中cd2＋的浓度分别为0、1、25、100μmol/l.在处理后的1、3、6、12、24h测定其快速上升荧光曲线和荧光参数.实验发现，高浓度的nacl（≥25mmol/l）和cd（≥25μmol/l）会降低psⅱ的活性，降低反应中心密度.nacl主要抑制了依赖于光的反应，降低了光能捕获和用于电子传递的量子产额.cd主要作用于反应中心，增加了单位反应中心吸收的光能和用于热耗散的能量.较低浓度（1μmol/l）的cd处理对小球藻psⅱ活性具有促进作用.nacl和cd的处理均体现了时间和浓度的依赖性.盐胁迫和cd胁迫体现了随浓度增高加重彼此胁迫强度的协同作用的特点.图7表1参28
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