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不同强度冷刺激对大鼠心脏、大脑、睾丸和肺脏中cirp表达的影响

DOI: 10.3724/SP.J.1145.2012.00055, PP. 55-59

Keywords: wistar大鼠,冷刺激,冷诱导rna结合蛋白表达,组织保护特异性,western-blot,实时荧光定量rt-pcr

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Abstract:

采用westernblot和实时荧光定量rt-pcr方法,研究不同强度不同时间冷刺激后大鼠心脏、大脑、睾丸和肺脏中冷诱导rna结合蛋白(cirp)的表达情况.结果表明:常温(20℃±1℃)下大鼠4种组织中均存在一定的cirp的表达;急性冷刺激组(-10℃±1℃、-4℃±1℃、0℃±1℃、4℃±1℃)心脏、大脑和睾丸中cirpmrna表达在冷刺激0~6h呈上升趋势,6h时达到最高值,随后呈下降趋势,肺脏中cirpmrna表达在0~6h呈下降趋势,在6h时达到最低值,随后呈上升趋势;慢性冷应激组(10℃±1℃)大鼠心脏、大脑和睾丸3种组织中cirpmrna在不同时间点(1周、2周、3w周)的表达均高于正常对照组(p>0.05),肺脏cirpmrna表达均低于对照组(p>0.05),并且同一组织不同时间点之间差异不显著(p>0.05).可见冷刺激后cirp的表达存在一定规律并且表现出组织特异性,可能与组织保护特异性有关.图2表2参15

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