OALib Journal期刊
ISSN: 2333-9721
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不同强度冷刺激对大鼠心脏、大脑、睾丸和肺脏中cirp表达的影响
DOI: 10.3724/SP.J.1145.2012.00055, PP. 55-59
Keywords: wistar大鼠,冷刺激,冷诱导rna结合蛋白表达,组织保护特异性,western-blot,实时荧光定量rt-pcr
Abstract:
采用westernblot和实时荧光定量rt-pcr方法,研究不同强度不同时间冷刺激后大鼠心脏、大脑、睾丸和肺脏中冷诱导rna结合蛋白(cirp)的表达情况.结果表明:常温(20℃±1℃)下大鼠4种组织中均存在一定的cirp的表达;急性冷刺激组(-10℃±1℃、-4℃±1℃、0℃±1℃、4℃±1℃)心脏、大脑和睾丸中cirpmrna表达在冷刺激0~6h呈上升趋势,6h时达到最高值,随后呈下降趋势,肺脏中cirpmrna表达在0~6h呈下降趋势,在6h时达到最低值,随后呈上升趋势;慢性冷应激组(10℃±1℃)大鼠心脏、大脑和睾丸3种组织中cirpmrna在不同时间点(1周、2周、3w周)的表达均高于正常对照组(p>0.05),肺脏cirpmrna表达均低于对照组(p>0.05),并且同一组织不同时间点之间差异不显著(p>0.05).可见冷刺激后cirp的表达存在一定规律并且表现出组织特异性,可能与组织保护特异性有关.图2表2参15
References
[1] | 2nishiyamah,itohk,kanekoy.aglycine-richrna-bindingproteinmediatingcoldinduciblesuppressionofmammaliancellgrowth.jcellbiol,1997,137(4):899~908
|
[2] | 4zhaoqx(赵巧香),zhangxl(张秀玲),jill(纪丽丽).preparationandidentificationofrecombinantproteinofcirp’spolyclonalantibodyinbalb/cmice.chinjapplenvironbiol(应用与环境生物学报),2009,15(5):634~637
|
[3] | 13nishiyamah,dannos,kanekoy.decreasedexpressionofcold-induciblerna-bindingprotein(cirp)inmalegermcellsatelevatedtemperature.am.jpathol,1998,152(1):289~296
|
[4] | 14bankss,kingsa,irvineds.impactofamildscrotalheatstressondnaintegrityinmurinespermatozoa.reproduction,2005,129(4):505~514
|
[5] | 1aloiarc,raisonjk.membranefunctioninmammalianhibernation.biochimbiophysacta,1989,988(1):123~146
|
[6] | 3nishiyamah,higashitsujih,yokoih.cloningandcharacterizationofhumancirp(cold-induciblerna-bindingprotein)cdnaandchromosomalassignmentofthegene.gene,1997,204(1~2):115~120
|
[7] | 5hongjk,kimyg,yoonsk.down-regulationofcold-induciblerna-bindingproteindoesnotimprovehypothermicgrowthofchinesehamsterovarycellsproducingerythropoietin.metabengin,2007,9(2):208~216
|
[8] | 6sakurait,itohk,higashitsujih.cirpprotectsagainsttumornecrosisfactor-α-inducedapoptosisviaactivationofextracellularsignal-regulatedkinase.biochimbiophysacta,2006,1763(3):290~295
|
[9] | 7hamidaa,mandaim,fujitaj.expressionofcold-induciblerna-bindingproteininthenormalendometrium,endometrialhyperplasia,andendometrialcarcinoma.jgynecolpathol,2003,22(3):240~247
|
[10] | 8liuaj(刘爱军),xueqh(薛菁晖),zhangzw(张志文).expressionofcold-induciblerna-bindingproteinmrnaintherat’sbrainunderhypothermia.chinjneuromedicline,2007,6(12):1228~1231
|
[11] | 9xuejh,nonoguchik,fukumotom.effectsofischemiaandh2o2onthecoldstressproteincirpexpressioninratneuronalcells.freeradicalbiol&med,1999,27(11~12):1238~1244
|
[12] | 10saitot,sugimotok,adachiy.cloningandcharacterizationofamphibiancoldinduciblerna-bindingprotein.compbiochemphysiolbbiochemmolbiol,2000,125(2):237~245
|
[13] | 11yinw(尹位).expressionofcirpfromthebalb/cmouseinnin3t3cellsandstudyofcold-inducedapoptopsis:[masterdegreedissertation].heilongjiangaugustfirstlandreclamationuniversity,daqing,china,2009
|
[14] | 12burdcg,dreyfussg.conservedstructureanddiversityoffunctionsofrna-bindingproteins.science,1994,265(5172):615~621
|
[15] | 15zhoukw,zhengxm,yangzw.overexpressionofcirpmayreducetesticulardamageinducedbycryptorchidism.clininvestmed,2009,32(2):e103~111
|
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