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OALib Journal期刊
ISSN: 2333-9721
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利用果胶培养基分离番茄根际细菌
DOI: 10.3724/SP.J.1145.2012.00147, PP. 147-152
Keywords: 果胶,培养基,番茄根际细菌,微生物分离,相似性系数,平板分离,dgge
Abstract:
采用平板稀释和pcr-dgge相结合的方法，比较了4种培养基（果胶富营养培养基pm，只含果胶一种营养的培养基pa，添加果胶的寡营养培养基ypp以及与ypp营养成分一致但不添加果胶的寡营养培养基ypg）分离番茄根际细菌的能力.结果显示：pa培养基能够分离到42种形态的细菌；ypp培养基可以分离到最高的细菌菌落数，分离获得的种类比pa少；ypp培养基分离获得细菌的种类和菌落数量比ypg培养基要多.聚类分析也显示只有果胶一种营养的pa培养基能分离到最多种类，分离获得的菌群与自然环境中微生物群落最相似.研究结果表明添加番茄根际主要分泌物――果胶到培养基中可以提高培养基分离细菌的能力.图3表1参20
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