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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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一株踝节霉菌的培养特性及其在污泥生物淋滤中的应用
DOI: 10.3724/SP.J.1145.2012.00128, PP. 128-133
Keywords: 踝节霉菌,嗜酸氧化亚铁硫杆菌,培养特性,hplc,污泥,生物淋滤
Abstract:
于实验室嗜酸氧化亚铁硫杆菌（acidithiobacillusferrooxidans）淋滤处理城市污泥酸性废液中分离出一株踝节霉菌zj-1，并对其进行了摇瓶液体培养特性研究.单因素试验结果表明：该菌株的最适碳源和氮源分别为葡萄糖和酵母膏，最适温度为28℃，最适转速为140r/min，最适初始ph值为5.0.正交试验优化得到碳、氮源的最优水平分别为葡萄糖70g/l，酵母膏15g/l.采用hplc对该菌株pda培养液中的有机酸进行了分析，发现其中含有丙酮酸.初始pda培养液中丙酮酸含量为0.039g/l，接菌培养d5.5时其含量达0.106g/l.为探讨踝节霉菌zj-1对嗜酸氧化亚铁硫杆菌淋滤污泥过程的影响，在生物淋滤起始阶段接种10%a.ferrooxidans和4%踝节霉菌zj-1，以仅接种10%a.ferrooxidans作为对照.结果表明a.ferrooxidans和踝节霉菌zj-1的复合菌组与对照组相比，淋滤耗时由7d缩短至4d，淋滤处理时间缩短了43%.图10表1参20
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