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OALib Journal期刊
ISSN: 2333-9721
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深黄伞形霉（umbelopsisisabellina）华2-1产油发酵培养条件优化
DOI: 10.3724/SP.J.1145.2012.00080, PP. 80-85
Keywords: 深黄伞形霉华2-1,微生物油脂,发酵条件优化
Abstract:
为开发微生物油脂资源，从富含油脂的土样中分离获得一株产油脂微生物――深黄伞形霉（umbelopsisisabellina）华2-1，油脂含量达48.60%.采用单因素和正交实验方法，对深黄伞形霉华2-1的发酵培养条件进行优化研究.优化的培养条件为：葡萄糖100g/l，酵母粉3g/l，接种量为20%，初始ph值为5.0~6.0，mgso4？7h2o和kh2po4的添加浓度分别为0.5g/l和2g/l，培养温度为31℃，最佳发酵培养时间为168h.华2-1在优化发酵条件下可获得菌体生物量、油脂产量和油脂含量分别为45.86g/l、24.47g/l和59.53%，较优化前分别提高了21.48%、33.42%和22.49%.因此，深黄伞形霉华2-1作为微生物油脂生产的新资源具有广阔的应用前景.图4表1参15
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