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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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porphyrobactersp.d22f的降解特性及其在石油降解菌群中的生态位
DOI: 10.3724/SP.J.1145.2012.00122, PP. 122-127
Keywords: 多环芳烃,产卟啉杆菌属,生物降解,起始双加氧酶,优势菌,生态位
Abstract:
为揭示多环芳烃（polycyclicaromatichydrocarbons，pahs）降解微生物资源的多样性和石油降解菌群的优势菌，研究了从南海沉积物中分离得到的一株pahs降解菌d22f的降解特性及其在石油降解菌群d22-1中的生态位.对菌株d22f进行16srrna基因同源性分析及透射电镜观察以初步确定其种属，通过培养法、气相色谱质谱联用（gc-ms）测定其多环芳烃降解范围和降解率，通过简并引物pcr扩增其pahs双加氧酶大亚基基因片段并进行系统发育分析，采用变性梯度凝胶电泳（dgge）监测石油降解菌群中的优势菌.结果表明，与菌株d22f的16srrna基因相似度最高的模式株为产卟啉杆菌属porphyrobactertepidariusdsm10594t（af465839；98.55%）.该菌株能降解萘、甲基萘、苊、硫芴、菲、蒽等；对初始浓度为0.2g/l菲10d后的降解率可达90%以上.从其基因组dna中克隆到的pahs起始双加氧酶大亚基基因phnac与novosphingobiumaromaticivoransdsm12444中的质粒pnl1（cp000676）上的bpha1f基因相似度最高，达到99.41%.dgge谱图显示，菌株d22f是石油降解菌群中的3种优势菌之一，在传代菌群中可稳定存在.porphyrobactersp.d22f为产卟啉杆菌属（porphyrobacter）中首株以低分子量pahs为唯一碳源和能源的菌株，是石油降解菌群的优势菌.图4表1参21
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