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OALib Journal期刊
ISSN: 2333-9721
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黑胸散白蚁纤维素酶的体外酶学特性
DOI: 10.3724/SP.J.1145.2012.00070, PP. 70-74
Keywords: 黑胸散白蚁,纤维素酶,内切β-1,4-葡聚糖酶,纤维二糖水解酶,β-葡萄糖苷酶,酶活,native-page
Abstract:
采用dns法研究了我国广泛分布的一种低等木食性白蚁――黑胸散白蚁纤维素酶的体外酶活特性以了解其纤维素降解机制.结果表明，内切β-1,4-葡聚糖酶（eg）、纤维二糖水解酶（cbh）和β-葡萄糖苷酶（bg）这3种酶的最佳反应时间均为15min，最佳底物浓度为1%，最适反应ph为5.6，最适反应温度为35℃.在最适反应条件下，eg、cbh和bg的活性分别达到71.3(±13.9)u/mg、5.8(±0.8)u/mg和4.1(±0.7)u/mg.eg在体外的热稳定性较差，在50℃及更高温度酶活很低或完全失活，但该酶对ph稳定性较好，在ph3.2~8.0范围内酶活力变化不大.native-page电泳检测到该白蚁体内至少有8种不同的eg活性条带，肠道不同部位纤维素酶活性条带种类不同.这些研究表明，木食性白蚁降解纤维素是一个复杂的过程，需要多种纤维素酶的共同作用.图8参17
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