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OALib Journal期刊
ISSN: 2333-9721
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budc缺失对克雷伯氏菌联产3-羟基丙酸和1,3-丙二醇的影响
DOI: 10.3724/SP.J.1145.2014.12031, PP. 371-376
Keywords: 3-羟基丙酸,1,3-丙二醇,budc缺失,克雷伯氏菌,醛脱氢酶
Abstract:
在利用克雷伯氏菌（klebsiellapneumoniae）联产3-羟基丙酸和1,3-丙二醇过程中，副产物对碳流的竞争是限制产物合成的重要因素.通过将来源于大肠杆菌的醛脱氢酶基因在budc缺失型克雷伯氏菌中过表达，研究budc缺失对克雷伯氏菌联产1,3-丙二醇和3-羟基丙酸的影响.与k.pneumoniaezg27/puc19-aldh相比，缺失型菌株k.pneumoniaezg40(budcd)/puc19-aldh发酵60h后，1,3-丙二醇和3-羟基丙酸的产量分别为64.0mmol/l和134mmol/l，3-羟基丙酸转化率提高了22%，副产物中除2,3-丁二醇外均有不同程度降低.本研究表明，过表达醛脱氢酶的重组克雷伯氏菌能够积累3-羟基丙酸，但在微氧发酵24h后，1,3-丙二醇逐渐向3-羟基丙酸转化；而budc的缺失进一步促进了1,3-丙二醇向3-羟基丙酸的转化.
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