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OALib Journal期刊
ISSN: 2333-9721
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高灵敏度铜抗性酿酒酵母表达系统的构建与应用
DOI: 10.3724/SP.J.1145.2014.12033, PP. 357-362
Keywords: 酿酒酵母,pcr-mediatedtechnique,cup1,铜抗性,绿色荧光蛋白
Abstract:
利用pcr-mediatedtechnique对酿酒酵母w303-1a金属硫蛋白基因（cup1）进行敲除，获得一株铜离子敏感型酿酒酵母（saccharomycescerevisiaew303-1acup1d），其对cu2+抑制浓度由1.2mmol/l降为0.08mmol/l；以pyx212载体为基本构架，以s.cerevisiaew303-1acup1d为宿主菌，构建了以cup1作为筛选标记的pyx212m表达系统，并成功表达了绿色荧光蛋白基因（gfp），从而实现了高灵敏度铜抗性酿酒酵母表达系统的构建与应用.本研究构建的高灵敏度铜抗性酿酒酵母表达系统不仅廉价、安全，而且丰富了酵母的表达系统，同时对微生物的铜抗性机理及生物修复功能的研究具有指导意义.
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