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OALib Journal期刊
ISSN: 2333-9721
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一株黑曲霉的分离鉴定及其对土壤重金属的生物浸出效果
DOI: 10.3724/SP.J.1145.2014.11019, PP. 420-425
Keywords: 黑曲霉,重金属污染土壤,有机酸,微生物沥浸
Abstract:
某些真菌可产生小分子有机酸，既具有酸化环境介质的功能，又具有络合重金属能力，因此可有效浸出环境介质（如土壤、底泥等）中的重金属.本研究从土壤中分离得到一株产酸能力较高的真菌，经形态学及itsrdna基因序列分析鉴定为黑曲霉，命名为aspergillusnigery9.测定了其生长特性，并采用摇瓶培养法探究其同步、分步培养法去除污染土壤中重金属的效果.结果显示，该菌适宜生长ph范围较广（3.0-8.0），对zn2+、cd2+、pb2+的80%抑制浓度分别为250、130、2700mg/l.培养20d后ph降至2.1，柠檬酸、葡萄糖酸、草酸和苹果酸的产量分别为54.8、20.1、15.9、6.3mmol/l.分步培养法污染土壤中重金属的浸出率最高为zn75%，cd100%，pb43%，略优于同步培养法（zn64%，cd86%，pb19%）.上述结果表明，aspergillusnigery9具有较强的重金属耐受性，能有效浸出土壤中的zn、cd、pb，为后期采用该菌处理重金属污染土壤提供了技术参考.
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