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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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北黄海近岸海域磺胺类抗生素及其抗性escherichiacoli分布
DOI: 10.3724/SP.J.1145.2014.11039, PP. 401-406
Keywords: 北黄海,磺胺类抗生素,抗性菌株,抗性水平
Abstract:
致病菌耐药性的增加和扩散目前已成为全球公共安全问题，为提供解决该问题的重要理论数据，在我国北黄海近岸海域采集排污口、海水养殖区和海滨浴场等水样和沉积物样品，利用hplc-ms/ms分析水体样品中14种磺胺类（sas）抗生素浓度含量，同时根据epa方法（method1604）对水和沉积物中escherichiacoli（e.coli）和磺胺抗性e.coli（re-e.coli）总量进行测定，计算出e.coli磺胺抗性水平，进而探讨该区域水体中e.coli磺胺抗性率与磺胺类抗生素浓度含量的相关性以及e.coli磺胺抗性菌株的分布特点及来源.结果显示，北黄海近岸海域水体中抗生素检出浓度差异性较大，磺胺浓度含量范围为nd-584.32ng/l；e.coli和re-e.coli每100ml总量范围在27×104-5.5×104和8×104-1.6×104株，在每克沉积物中分别为0-1363和0-320株；e.coli磺胺抗性率范围为18.18%-66.91%.研究表明，e.coli磺胺抗性率与磺胺类抗生素浓度含量存在显著相关性（p=0.846），说明环境残留抗生素可诱导抗性微生物；e.coli和re-e.coli分布和抗性水平显示，抗性基因之间存在水平转移，且其主要来源是渔业养殖.
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