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OALib Journal期刊
ISSN: 2333-9721
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基于连续发酵驯化的高耐盐性酿酒酵母的育种
DOI: 10.3724/SP.J.1145.2014.11032, PP. 360-370
Keywords: 酿酒酵母,耐盐性,乙醇连续发酵,四孢子分析,燃料乙醇,海藻糖
Abstract:
耐盐酿酒酵母菌株的育种对降低燃料乙醇生产成本具有重要意义.以具有优秀乙醇发酵能力的工业酿酒母菌株kf-7为出发菌株，通过连续发酵、产孢子及孢子培育、交配等获取稳定耐盐菌株.在盐胁迫条件下利用连续乙醇发酵驯化获得了耐盐突变菌株kf-7(4).在此基础上，通过孢子分离、培养、评价和交配，获得两株耐盐二倍体菌株kf-7(4)-3与kf-7-d1.这3株耐盐菌株在50次转接过程中保持着稳定的耐盐性.并且在9%kcl浓度下，3株耐盐菌株的乙醇发酵能力显著优于出发菌株kf-7：在15%ypd培养基中，发酵36h时的乙醇浓度比出发菌株kf-7提高了21%.有盐和无盐条件下发酵过程中胞内海藻糖含量分析表明，突变菌株kf-7(4)和菌株kf-7(4)-3即使在无盐条件下的海藻糖积累能力明显高于出发菌株kf-7.本研究获得的变异酿酒酵母菌株具有较高的耐盐性和稳定性，耐盐性与胞内海藻糖积累能力提高相关.因此，基于连续发酵的进化工程手段可以有效地用于培育具有某种稳定性状的酿酒酵母菌株.
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