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OALib Journal期刊
ISSN: 2333-9721
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烷烃降解菌的筛选、鉴定及优势菌株的降解特性
DOI: 10.3724/SP.J.1145.2011.00572, PP. 572-576
Keywords: 烷烃降解,红球菌,菌株筛选,16srdna,石油污染土壤,生物修复
Abstract:
以正庚烷为唯一碳源，从长期受到石油污染的土壤中筛选获得可利用正庚烷的微生物14株.通过形态观察和16srdna序列比对，鉴定g2、g9、g14为红球菌属，g3、g27为人苍白杆菌属，g4、g7为芽孢杆菌属，g5、g10、g15、g25为节杆菌属，g16为缺陷短波单胞菌，g17、g22为嗜麦芽寡养单胞菌属.通过考察其降解烷烃的能力，确定rhodococcussp.g2为烷烃降解优势菌株.该菌株可代谢庚烷获得最大菌体浓度d600nm=7.51.同时该菌对不同碳链长度的烷烃，如十二烷、十六烷、煤油和二甲苯均具有较强的降解能力，以十二烷为碳源的最大比生长速率为0.37h-1，最高菌体浓度为d600nm=12.00，在正十六烷中生长，最大比生长速率为0.23h-1，在煤油中生长，最大比生长速率为0.14h-1，在以二甲苯为唯一碳源时，d600nm也可达到1.00左右.研究表明该菌株对于石油污染土壤的生物修复有很大的应用前景.图6表2参9
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