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OALib Journal期刊
ISSN: 2333-9721
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拟南芥硫酯酶基因（atfata）在大肠杆菌中的表达及其对游离脂肪酸合成的影响
DOI: 10.3724/SP.J.1145.2011.00568, PP. 568-571
Keywords: 拟南芥,硫脂酶,工程大肠杆菌,胞外游离脂肪酸
Abstract:
硫酯酶催化脂酰acp（酰基载体蛋白）水解生成游离脂肪酸和acp载体蛋白，能够解除脂肪酸合成与磷脂合成的偶联，是获得游离脂肪酸的关键基因.将拟南芥的硫酯酶基因atfata克隆到原核表达载体pet30a上，在大肠杆菌bl21(de3)中成功表达了his-tag融合蛋白，经sds-page检测获得特异性表达条带.对该重组菌株进行摇瓶发酵实验，gc-ms检测表明其与对照菌株脂肪酸组成并未发生变化；但其脂肪酸产量达到232.06mg/l，比对照菌株提高了70%；同时胞外游离脂肪酸产量达到了33mg/l，占总脂肪酸含量的15%，是野生菌株胞外脂肪酸产量的7倍.图3表2参17
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