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OALib Journal期刊
ISSN: 2333-9721
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重组大肠杆菌产cota漆酶的发酵条件优化
DOI: 10.3724/SP.J.1145.2011.00563, PP. 563-567
Keywords: cota漆酶,大肠杆菌,重组工程菌,发酵条件优化
Abstract:
cota漆酶在环境保护、食品工业和纸浆漂白等工业中具有重要的应用价值.将重组表达载体pet-22b/cota转入大肠杆菌bl21（de3），得到工程菌株，采用单因素实验和正交实验相结合的方法，研究诱导表达条件和发酵培养基对重组大肠杆菌产cota漆酶量的影响.结果表明，初始ph7.5的培养基中，添加0.6mmoll-1cu2+，1gl-1葡萄糖作碳源、15gl-1蛋白胨和2gl-1硫酸铵作氮源，以10%的接种量，37℃、200r/min，直到菌液的d600nm值为1.0，加入终浓度为1.0mmoll-1的iptg，25℃诱导12h，漆酶的产量最高.优化前发酵液的粗提液的漆酶活性仅为1190uml-1，优化后达到3526uml-1，正交实验优化后漆酶活性提高了2.96倍.纯化的cota漆酶最适反应温度为45℃，最适ph值为7.2.cota漆酶对rbbr的脱色率在90%以上，此cota漆酶在短时间内对染料能够有效地脱色，能够成为有潜力的工业用酶.图6表3参17
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