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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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华重楼内生菌iun35的分离及其抗菌蛋白的性质
DOI: 10.3724/SP.J.1145.2011.00537, PP. 537-540
Keywords: 华重楼,内生细菌,抗菌蛋白,分离纯化,抑菌活性
Abstract:
从华重楼（parispolyphyllasmithvar.chinensis）块茎中分离纯化得到一株具有较强抑菌活性的内生细菌iun35，经形态特征、生理生化特性及16srdna鉴定为枯草芽孢杆菌（bacillussubtilis）.将分离得到的iun35菌株发酵培养，培养液上清经硫酸铵分级沉淀、sephadexg-75凝胶柱层析和deae-32纤维素柱层析分离纯化出一种抗菌蛋白ud35.用该蛋白对多种植物病原菌进行拮抗测定，结果显示，ud35对玉米纹枯病菌（rhizoctoniasolanikuha）、小麦赤霉病菌[gibberellezeae（schw.）]等多种菌有强烈抑制作用.测定其相对分子质量约为55000，氨基酸分析结果表明其由17种氨基酸组成.图3表4参16
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