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OALib Journal期刊
ISSN: 2333-9721
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没食子酸丙酯对k562细胞的增殖抑制和诱导凋亡作用
DOI: 10.3724/SP.J.1145.2011.00517, PP. 517-520
Keywords: 没食子酸丙酯（pg）,抗肿瘤,乳酸脱氢酶（ldh）,k562细胞,增殖抑制,细胞凋亡,赤芍,中药
Abstract:
观察没食子酸丙酯（propylgallate，pg）对人慢性髓系白血病k562细胞株的增殖抑制及诱导凋亡作用.采用倒置显微镜观察细胞形态及数目的变化，乳酸脱氢酶（lactatedehydrogenase，ldh）释放检测细胞活性，流式细胞术pi染色法及dna倍体分析检测细胞凋亡作用.结果表明，没食子酸丙酯能有效地抑制k562细胞增殖，药物处理24h时细胞形态发生变化，细胞数目减少；细胞培养液中ldh活性分析显示，20~300?g/ml没食子酸丙酯处理24h对细胞毒性作用不明显；但处理时间达48h时，没食子酸丙酯对细胞毒性明显增加；亚二倍体峰（凋亡峰）的出现及dna片段化分析表明，200?g/mlpg处理细胞24h时，能引起k562细胞凋亡.结果表明，pg具有明显的体外抗肿瘤活性，其抗癌活性与其抑制细胞增殖、诱导细胞凋亡有关.图5参18
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