|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
青枯菌不同色谱峰组分的细胞表面特性
DOI: 10.3724/SP.J.1145.2011.00548, PP. 548-552
Keywords: 青枯菌,细胞表面特性,胞外酸性多糖,高效离子交换色谱,色谱组分,活细胞
Abstract:
将高效离子交换色谱应用于植物重要致病菌――青枯菌的分离分析.青枯菌纯培养物经过色谱分离得到3个特征峰，收集3个色谱峰组分进行形态观察和生理生化鉴定，结果表明3个组分均属于青枯菌生化ⅲ型；进一步分析发现，3个色谱峰组分在质粒dna、细胞表面鞭毛菌毛蛋白含量和全细胞脂多糖成分方面无明显差异，但细胞表面黏附的胞外酸性多糖epsⅰ含量差异显著；3个色谱峰青枯菌细胞表面疏水性的测定结果也证实了epsⅰ是造成不同青枯菌细胞色谱行为差异的主要原因.高效离子交换色谱为细菌活细胞的研究提供了一种新方法.图4表2参15
| [1] | 1danielssl.theadsorptionofmicro-organismsontosolidsurface.devindmicrobiol,1972,13:211~223
|
| [2] | 2dicksonjs,koohmaraiem.cellsurfacechargecharacteristicsandtheirrelationshiptobacterialattachmenttomeatsurface.applenvironmicrobiol,1989,55(4):832~836
|
| [3] | 3linj(林娟),mac(马骋),liust(刘树滔),xiez(谢智),raopf(饶平凡).optimizationofralstoniasolanacearumpreparationmethodsbyhplcanalysis.chinabiotech(中国生物工程杂志),2006,26(5):63~68
|
| [4] | 4linj(林娟),raopf(饶平凡),mac(马骋),liust(刘树滔),xiez(谢智).studyontheseparatingconditionsofralstoniasolanacearumbyhighperformanceionexchangeliquidchromatographyanalysis.jfuzhouunivnatscied(福州大学学报自然科学版),2006,34(3):448~452
|
| [5] | 5linj(林娟),gaozn(高珍娜),liust(刘树滔),raopf(饶平凡).adsorptioncharacteristicsofcellsurfaceofralstoniasolanacearumwildtypestrainandavirulentmutant.chinjapplenvironbiol(应用与环境生物学报),2010,16(4):499~503
|
| [6] | 8charth.methodsinpracticallaboratorybacteriology.bocaraton,usa:crcpress,inc,1994.1~20
|
| [7] | 9linj(林娟),mac(马骋),liust(刘树滔),raopf(饶平凡).charaterizationofralstoniasolanacearumindifferentgrowthphasesbyhighperformanceionexchangeliquidchromatographyanalysis.actamicrobiolsin(微生物学报),2007,47(1):145~149
|
| [8] | 10ahimouf,paquotm,jacquesp,thonartp,rouxhetpg.influenceofelectricalpropertiesontheevaluationofthesurfacehydrophobicityofbacillussubtilis.jmicrobiolmethods,2001,45(2):119~126
|
| [9] | 12linj(林娟),liust(刘树滔),gaozn(高珍娜),raopf(饶平凡).analysisofdifferentvirulentralstoniasolanacearumstrainsusinghighperformanceionexchangechromatography.chinjapplenvironbiol(应用与环境生物学报),2009,15(5):713~718
|
| [10] | 13zhoudq(周德庆).microbiology.2nded.beijing,china:highereducationpress(北京:高等教育出版社),2002.11~16
|
| [11] | 14orgambideg,montrozierh,servinp,rousselj,trigaletdd,trigaleta.highheterogeneityoftheexopolysaccharidesofpseudomonassolanacearumstaingmi1000andcompletestructureofthemajorpolysaccharide.jbiolchem,1991,266(13):8312~8321
|
| [12] | 15wilsonww,wademm,holmansc,champlinfr.statusofmethodsforassessingbacterialcellsurfacechargepropertiesbasedonzetapotentialmeasurements.jmicrobiolmethods,2001,43(3):153~164
|
| [13] | 6llopp,carusop,cuberoj,morentec,lopezmm.asimpleextractionprocedureforefficientroutinedetectionofpathogenicbacteriainplantmaterialbypolymerasechainreaction.jmicrobiolmethods,1999,37(1):23~31
|
| [14] | 7zhaoyf.principleandapplicationofbiochemistry.3rded.beijing,china:sciencepress(北京:科学出版社),2002.308~314
|
| [15] | 11zhangy(张洋),went(温腾),linj(林娟),xiez(谢智),liust(刘树滔),raopf(饶平凡).characterizationandidentificationofralstoniasolanacearumbyion-exchangechromatography.chinjchromatogr(色谱),2005,23(4):418~421
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
