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麻疯树种子中佛波酯的提取及对菜青虫的杀虫活性

DOI: 10.3724/SP.J.1145.2011.00532, PP. 532-536

Keywords: 麻疯树,佛波酯,hplc,菜青虫,杀虫活性,拒食活性

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Abstract:

佛波酯是麻疯树种子中的主要毒性物质之一.麻疯树种子经乙醇浸提、液液萃取和柱层析,获得佛波酯富集物.以tpa为外标,通过高效液相色谱(hplc)定量分析佛波酯的含量,同时检测其对菜青虫的毒杀和拒食活性.结果显示,富集物中佛波酯含量达到0.81735mgmg-1.佛波酯富集物对3龄菜青虫具有显著的毒杀和拒食活性:毒杀活性表现为胃毒作用,胃毒活性具有明显的剂量效应和时间效应,48、72、120h的佛波酯半致死浓度(lc50)分别为73.86、16.99、5.26μgml-1;24、48h的非选择拒食中浓度(afc50)分别为9.81、5.39μgml-1.图3表3参24

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