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太湖和巢湖夏季蓝藻水华期间产毒微囊藻和非产毒微囊藻种群丰度的空间分布

DOI: 10.3724/SP.J.1145.2011.00480, PP. 480-485

Keywords: 微囊藻,产毒微囊藻,种群丰度,荧光定量pcr,太湖,巢湖,蓝藻水华

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Abstract:

应用荧光定量pcr技术对蓝藻水华暴发期间太湖和巢湖水体中产毒微囊藻和总微囊藻种群丰度的空间分布进行了研究.分别以微囊藻毒素合成基因基因家族成员mcyd和小核糖体16srdna序列构建定量pcr标准曲线,研究产毒微囊藻和总微囊藻种群丰度.结果表明:太湖和巢湖微囊藻种群由产毒微囊藻和非产毒微囊藻组成;蓝藻水华暴发期间,微囊藻种群组成及其丰度分布具有明显的空间差异性:太湖产毒微囊藻种群丰度为9.89×104~4.51×106copiesml-1,产毒微囊藻占总微囊藻种群的比例为20.5%~38.1%;巢湖产毒微囊藻种群丰度为4.12×104~5.44×106copiesml-1,其占总微囊藻种群的比例为8.4%~96.6%.总的来说,富营养化严重的湖区总微囊藻和产毒微囊藻种群丰度较高,产毒微囊藻占总微囊藻种群的比例也较高.图7表3参29

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