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OALib Journal期刊
ISSN: 2333-9721
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应用tail-pcr扩增转基因蓝猪耳t-dna侧翼序列
DOI: 10.3724/SP.J.1145.2009.00871, PP. 871-874
Keywords: 蓝猪耳,tail-pcr,t-dna,整合位点,侧翼序列
Abstract:
蓝猪耳是一种重要的具有观赏和科研价值的花卉植物.采用tail-pcr成功扩增了转基因蓝猪耳t-dna插入位点的侧翼序列，扩增片断长度为200~2000bp，大多数片段在400bp和800bp左右，其中36%的序列含有植物的同源序列.通过与genbank数据库比对，确定了部分t-dna插入位点周边序列编码的可能蛋白，并提交序列7条；另外还对t-dna转化植物时整合的位点进行了分析，发现断裂位点集中在距右边界15~18bp和右边界外234bp处，分别占总扩增片段的47.62%和38.10%.这为利用t-dna标签进行蓝猪耳基因克隆和功能分析提供了实验技术上的保证.图2表2参24
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